There is a problem with the analysis given for Example 1 of ANOVA with Repeated Measures for One Within Subjects Factor, namely that the sphericity requirement for ANOVA with matched samples has not been met. In fact the requirements for ANOVA with matched samples are as follows:

- Subjects are independent and randomly selected from the population
- Normality (or at least symmetry) – although overly restrictive it is sufficient for this condition to be met for each treatment (i.e. each column in the analyses that we present)
- Sphericity

Sphericity is an assumption which is stronger than homogeneity of variances, and is defined as follows.

**Definition 1**: Suppose you have *k* samples of equal size. The data has the **sphericity** property when the pairwise differences in variance between the samples are all equal.

Such data has the **compound symmetry** property when the all variances are equal and all the covariances are equal.

**Observation**: Obviously if there are only two treatments then sphericity is automatically satisfied.

**Property 1**: Compound symmetry implies sphericity

Proof: Suppose that the data satisfies the compound symmetry property. We consider the case with *k *= 3; the general case follows similarly. Then the covariance matrix has the form

This can then be simplified to

where *ρ* is the correlation coefficient. By Property 5 of Basic Concepts of Correlation

From which it follows that the data satisfies the sphericity property.

**Observation**: The converse is not true since it is easy enough to give a counter-example.

**Example 1**: Determine whether the data for Example 1 of ANOVA with Repeated Measures for One Within Subjects Factor (repeated in Figure 0) meets the sphericity assumption.

Since we have 4 treatments *T _{0}, T_{1}, T_{2} *and

*T*the covariance matrix

_{3}*S*is an array of form [

*c*] where

_{ij}*c*=

_{ij}*cov*(

*T*) for

_{i}, T_{j}*i ≠ j*and c

_{ij}=

*var*(

*T*) for

_{i}*i = j*. For this example

*S*is given in Figure 1.

The worksheet in Figure 1 can be created by highlighting the range W25:Z28 and entering the supplemental array formula =COV(H6:K20) described in Method of Least Squares for Multiple Regression (or the standard Excel techniques described following Figure 2 of Method of Least Squares for Multiple Regression) by referencing cells in the worksheet in Figure 0.

Clearly the variances (the values along the main diagonal) are different and the covariances are different, and so compound symmetry doesn’t strictly hold, but it is not clear whether these differences are significant.

Next we calculate the variances of the pairwise differences between the treatment values. This can either be calculated directly or by using Property 5 of Basic Concepts of Correlation that

The results for Example 1 are given in Figure 2. For example, cell AD6 is simply the difference between the score for subject 1 before the training and 1 week after (i.e. =H6-I6, referencing the cells in Figure 0) and cell AD22 can be calculated either as =VAR(AD6:AD20) or as =W25+X26-2*X25 (referencing the cells in Figure 1).

From Figure 2, we conclude that the variances of the 6 pairs are clearly not equal, and so it appears that the sphericity assumption has not been met. The resolution is to use a correction factor called **epsilon** to reduce the degrees of freedom in the test described in ANOVA with Repeated Measures for One Within Subjects Factor. The most common version of epsilon, due to **Greenhouse and Geisser**, takes a value between and 1 where *k* = the number of treatments (i.e. the number of columns, 4 in our example). The value epsilon is given by the formula:

Here *S* = the sample covariance matrix, = the mean of all the elements in *S*, = the mean of all the elements on the diagonal of (i.e. the mean of the variances). For our example, we have = 0.493 as calculated in cell AR12 of Figure 3 (with references to cells in the worksheet shown in Figure 0).

In general, is viewed as too conservative (i.e. the correction factor should be higher), and so another common correction factor called the **Huynh and Feldt epsilon** is also commonly used. It turns out that this factor tends to be slightly too high.

The Huynh and Feldt epsilon is calculated as follows where n = the number of subjects.

For our example, = 0.538, as calculated in cell AR19 of the worksheet in Figure 3.

There is one more commonly used correct factor, namely . This is the lower bound. The correction for sphericity can’t be lower than this.

**Real Statistics Functions**: The Real Statistics Resource Pack provides the following two functions which calculates the GG epsilon and HF epsilon correction factors for both the one factor and two factor repeated measures Anova.

**GGEpsilon**(R1, *ngroups*, *raw*) = Greenhouse and Geisser epsilon value for the data in range R1 where *ngroups *= the number of independent groups; if *raw *= TRUE then R1 contains raw data, otherwise it contains a covariance matrix

**HFEpsilon**(R1, *ngroups*, *nsubj*) = Huynh and Feldt epsilon value for the data in range R1 where *ngroups *= the number of independent groups; if *nsubj *= 0 then R1 contains raw data, otherwise it contains a covariance matrix which is derived from raw data withnsubj subjects (corresponding to rows).

For the one factor case *ngroups* = 1. For Example 1, referring to Figure 0, we see that GGEpsilon(H6:K20) = .493 and HFEpsilon(H6:K20) = .538. Referring to Figure 1, we see that GGEpsilon(W25:Z28,1,FALSE) = .493 and HFEpsilon(W25:Z28,1,15) = .538.

**Example 2:** Revise the analysis for Example 1 by using the GG and HF epsilon correction factors.

We correct the column and error degrees of freedom by multiplying the *df _{Groups} *and

*df*values shown in Figure 2 of ANOVA with Repeated Measures for One Within Subjects Factor (and duplicated in the range N3:T8 of Figure 4 below) by an epsilon correction factor. E.g. the revised value of

_{E}*df*using the Greenhouse and Geisser epsilon is therefore

_{E}*df*∙ = 42 ∙ .493 = 20.7. These revised values of the degrees of freedom are then used to calculate new values of

_{E }*MS*and

_{Groups}*MS*, and then

_{E}*F*=

*MS*/

_{Groups}*MS*. Note that the revised degrees of freedom won’t necessarily be a whole number. The results are summarized in Figure 4.

_{E}**Observation**: Since Excel only uses whole number degrees of freedom, the actual values used in the above calculations are rounded down to the nearest whole number, i.e. *df _{Col }*= 1 and

*df*= 22. You may need to interpolate to get slightly more accurate results. The above analysis shows that the null hypothesis should still be rejected (

_{E}*p*< .05 =

*α*).

**Real Statistics Data Analysis Tool**: The **One Factor Repeated Measures Anova** supplemental data analysis tool contained in the Real Statistics Resource Pack can be used to automatically perform analysis of variance for repeated measures, including the calculation of the GG and HF epsilon correction factors.

For Example 2, enter **Ctrl-m** and double click on **Analysis of Variance** (as shown Figure 0 of Anova Confidence Interval). Next select **Repeated Measures Anova: one factor **from the dialog box that appears.** **A dialog box will now appear as shown in Figure 5.

**Figure 5 – Dialog box for Repeated Measures Anova**

Fill in the dialog box as shown in the figure and click on **OK**. The output is as shown in Figure 6.

**Figure 6 – One Factor Repeated Measures Anova**

**Observation**: A rule of thumb is that if sphericity is greater than .75 then the Huynh and Feldt epsilon should be used; otherwise the Greenhouse and Geisser epsilon should be used. When sphericity is very low, it might be better to use MANOVA since it does not rely on the sphericity assumption. A rule of thumb is that for sphericity < .7 you should use MANOVA provided your sample size is at least 10 + *k*; otherwise you should use ANOVA.

**Observation**: For more information about sphericity, including other ways of calculating Greenhouse and Geisser epsilon, as well as Machly’s test for sphericity, click here.

**Observation**: We can also calculate an effect size for the omnibus ANOVA. This is omega squared, although the formula used is different from the one used for ANOVA with independent variables.

For Example 2, *ω ^{2}* = .14 using the GG epsilon correction factor. As we have noted elsewhere, the effect size for comparisons is more meaningful than the effect size of the omnibus ANOVA.

Dear Charles,

Thank you for your informative and very practical web site. My question relates to sphericity tests applied to two way ANOVAs. For the single factors it is clear the same calculations would apply as for the one way case, but what about calculating sphericity for the interaction term? Is the correct covariance matrix one that consists of all values of both factors?

Regards,

James

James,

I think you are referring to the case described on the following webpage:

http://www.real-statistics.com/anova-repeated-measures/one-between-subjects-factor-and-one-within-subjects-factor/

Charles

Dear Charles!

I have a question with regards to the “One repeated measures procedure”.

Assume, I would like to compare overnights of 100 different hotels between the years 1999, 2000, and 2001. The typical way to check whether sphericity can be assumed – at least to my knowledge – is first to run Mauchly’s test of sphericity or John, Nagao and Sugiura’s test of sphericity. If this test is significant, we have a look at the Greenhouse-Geisser epsilon to decide upon whether to apply the Greenhouse-Geisser or Huyhn-Feldt correction (the .75 rule).

My question is, how to quickly check with the Realstat add-ins whether the sphericity assumption in such a case is violated, as the “One repeated measures procedure” implemented in the Realstat add-ins does not give me information about sphericity, or does it?

Many thanks in advance for your response!

Best wishes,

Christian

Christian,

The Real Statistics add-in does provide information about sphericity. See, for example, Figure 6 on the referenced webpage.

Also, you don’t really need to test for sphericity using Mauchly’s test or John, Nagao, Sugiura’s test. You can simply apply the GG or HF correction.

Charles

Dear Dr. Charles,

first of all thank you very much for all the help you provide us through your website and statistical tools for excel!

I would like to have your comments about this question.

I have a one-factor repeated measures ANOVA, related to clinical data from a group of subjects that have been measured before receiving a treatment and then measured again after the treatment, for 7 times during a given time period.

The 8 groups of data show a relevant deviation from sphericity (GGepsilon = 0.54, HFepsilon = 0.59).

Anyway, after applying the GG or HF correction, the ANOVA resulted significant (p = 0.022) so I wish to proceed with post-hoc comparisons.

I am not interested to all pairwise comparison, but only in comparing pre-treatment group with each post-treatment group, so a total of 7 not-orthogonal contrasts.

Given that the sphericity assumption is largely violated, can I still perform paired t-test between groups of interest with the Bonferroni correction (t-test alpha = 0.05/7), or this is not recommended?

I tried to search on the web, but it seems that there is not good agreement about this issue, so I would appreciate to have your opinion about.

Thank you very much

Piero

Piero,

Since there is no sphericity issue for pairwise tests, I can’t think of any reason why you couldn’t do exactly what you propose (or use the Dunn/Sidak correction).

Charles

Dear Charles

Thank you very much for your help and time, it’s very appreciated as well as the precious work you do with this website and statistic tools of yours! I wouldn’t know what to do without it!

I didn’t make myself clear very much with my question about the test but I think I figured it out – next thing I have to do with my data is do a Tukey’s HSD test. However, I am not quite sure, therefore you may can help me with this:

I have a study where the teeth of 40 subjects were measured over time 5 times (repeated measures in the same subjects) and the L (ligthness), b (yellow – blue colour), a (green – red colour), C (colour intensity) and h (hue) values are recorded. From the start of the study/analysis I know that there will be no change in the h and a values – so these parameters are not interesting for me, only the L, b and C value I would like to analyse closer. Now I thought I would do the analysis the following way: perform with every parameter (L, b, C) seperately an ANOVA repeated measure with one within subject factor analysis followed by a Tukey’s HSD test with Bonferroni correction. Is that a good approach? Thank you very much for your help – statistics is really difficult for non-statisticans!

Claudine,

I agree that statistics can be difficult, not just for non-statisticians, but for everyone. Since you have three dependent variables, it sounds like you might benefit from using MANOVA (the multivariate analog to ANOVA). This will take into account any correlations between the dependent variables. You can read more about this on the webpage MANOVA, especially Two Factor Multivariate Repeated Measures.

Charles

Dear Charles

One more question: and then what test do I need to do, to find out between which groups there is a significant difference?

Thanks a lot for your help!

Claudine

Claudine,

I don’t know how to calculate whether there is a significant difference between the groups; if a significance test is required you should use Mauchly’s test. However, as I said in my previous response, generally it is better to use the GG or HF correction factor. Another rule of thumb here is that if the epsilon-GG < .75 use the GG correction; otherwise use the HF correction. Charles

Dear Charles

Thank you very much for your Analysis Tool and explanations – it’s very helpful!

I used it do to a one within subjects factor analysis where I have subjects that were measured over time 5 times and got an output similar to figure 6. What I don’t understand is how I see out of the output data whether sphericity is meet or not – and therefore which data I have to report, df-corrected (GG; HF) or the not corrected Anova data? Is there a rule or easy way to see it? Do I have to do a “manual” analysis like shown in figure 2 and if so then what “difference” between the pairwise differences in variance between samples is supposed to be fair enough to meet the sphericity property of “equal”?

Dear Claudine,

Generally, it is better to simply use either the GG or HF correction factor and not worry about whether the sphericity assumption is met or not. Of course if the corrected test values are pretty similar to the uncorrected values then probably sphericity wasn’t much of a problem. Regarding which correction factor to use, some people use GG and some use an average between GG and HF. Additional information is given on the referenced webpage.

If you need to calculate the sphericity you can manually perform the calculations shown in Figure 2. Alternatively you can use Mauchly’s test as described in Additional Information about Sphericity.

Charles